ANSC 4001 Chapter : 2017 Cattle Parasites Lab 2
Document Summary
Louisiana state university school of veterinary medicine. Procedure: add 30-40ml of soapy solution to 2 grams fecal material and mix well. This can be done in the 50ml tube the feces is in. Strain fecal solution through sieve into the small beaker: carefully pour strained material back into 50 ml centrifuge tube. Fill the tube with soapy solution to the 45-50ml line. Procedure cont: carefully and continuously pour off supernate until meniscus reaches the bottom of the tube, once you start to pour do not stop and. Tilt tube upright (~10-15ml remain) and add more soapy solution to the 45-50ml line. Let it sit for 3 minutes: at this point determine how many more washes need to be done. Procedure cont: after final pour off, transfer contents into a 15 ml tube, you can either pour or transfer with pipette, add 2 drops of methylene blue, using a pipette remove and discard all but 2ml of.
