BE 2352 Chapter : BE2352 Lab1
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Cell Membranes Lab Report
- Diffusion
- Compare the following terms: solvent, solute, andsolution.
- Go to Lab, Section I, Exercise 1 to explore the factorsinfluencing the rate of diffusion and complete the following:
- Predict how molecular mass and temperature may affect theoutcome of this experiment.
- Record the data from information in the Lab in the tablebelow.
Time (min) | Diameter (mm) | |||
5°C MB | 5°C PP | 23°CMB | 23°C PP | |
0 | ||||
15 | ||||
30 | ||||
45 |
- Construct a line graph of the data from the table above. Yourgraph should include labeled axes, units, and a legend indicatingwhich treatment is presented. Sign, date and prepare an image ofyour graph and include it with this lab report.
- Osmosis
- Go to Lab, Section II, Exercise 2 to view a demonstration ofosmosis and answer the following questions:
- Describe the net movement of water in osmometer 1.
- How is the movement of water molecules related to theconcentration gradient of the solution?
- Go to Lab, Section II, Exercise 3 to observe the effect ofsolute concentration on the rate of osmosis. Answer the followingquestions:
- Was the net movement of water in bags 1 to 4 into or out of thebags?
- Explain the results from bags 4 and 5.
- Selective Permeability of Membranes
- Go to Lab, Section III, Exercise 4 to learn about theimportance of selectively permeable membranes, then complete thetable and answer the following questions:
Contents of Beaker | ||
0 Min | 30 Min | |
Starch | ||
Chloride ion |
- Which substances diffused through the dialysis membrane?
- How does dialysis tubing model the selective permeability of aplasma membrane?
- Tonicity
- Go to Lab, Section IV, Exercise 5 and 6 to observe plasmolysisin Elodea cells and tonicity in red blood cells. Summarizethe concept of tonicity using blood and Elodea cells asexamples. Your description should incorporate these terms: turgid,plasmolysis, hemolysis, and crenation.
Cells | Isotonic Solution | Hypertonic Solution | Hypotonic Solution |
Elodea | |||
RBC |
- Based on your previous work, reproduce the table below and drawan illustration of a single cell in each box. Include arrows toshow the direction of water flow relative to each cell and dots tosymbolize solutes. Sign, date and prepare an image of your drawingand include it with this lab report.
Cells | Isotonic Solution | Hypertonic Solution | Hypotonic Solution |
Elodea | |||
RBC |
Summary Questions
- Compare diffusion and osmosis. Give an example of each.
2. In which direction will osmosisoccur if a 15% sugar solution is separated from a 25% sugarsolution by a selectively permeable membrane?
3. Why did osmosis, but not diffusionof sucrose molecules, occur across the dialysis membrane containing20% sucrose solution?
4. You are having a party and thecelery is limp. What might you do to make the celery crisp (turgid)again? What will occur in the cells?
5. A small amount of fertilizer(mineral salts) will stimulate plant growth, but over fertilizationcan kill plants? Why?
6. Michael adds sugar to his coffee.Explain what in this drink is the solvent, solute, and solution.
7. You add a cube of sugar to yourdrink. How could you speed up the diffusion of sugar moleculeswithout stirring?
8. How is dynamic equilibriumestablished within a solution?
HELP ON ECOLOGY LAB REPORT PLEASE!!!
- The AIM of this lab exercise: Does Eastern Mosquitofish (Gambusia holbrooki) population structure differ with season?
- You will compare population structure in an FIU pond from February 2018 (previous semester) with the data that you collect this semester (September 2018).
- CREATE HYPOTHESIS ABOUT POPULATION STRUCTURE (HELP PLEASE)
(HELP PLEASE) Include at least one paragraph ON THE key points of comparison between the life tables. Include the major results for population structure, even if no difference is found! Do these populations differ with respect to optimal age of sexual maturity, R0, G, and r?
FEBRUARY 2018 CURRENT RESULTS
Age Class (days) x | Sample/ Age s(x) | Number/ Age n(x) | Survival Rate 1(x) | Fecundity b(x) | Offspring/Ind 1(x)*b(x) | Age Weighted Fecundity 1(x)*b(x)*x |
0 | 24 | 120 | 1 | 0 | 0 | 0 |
30 | 39 | 96 | 0.8 | 0 | 0 | 0 |
60 | 19 | 57 | 0.475 | 0 | 0 | 0 |
90 | 21 | 38 | 0.317 | 24 | 7.608 | 684.72 |
120 | 8 | 17 | 0.142 | 27 | 3.834 | 460.08 |
150 | 5 | 9 | 0.075 | 29 | 2.175 | 326.25 |
180 | 2 | 4 | 0.033 | 32 | 1.056 | 190.08 |
210 | 0 | 2 | 0.017 | 0 | 0 | 0 |
240 | 1 | 2 | 0.017 | 0 | 0 | 0 |
Sum | 120 | R0 | 14.673 Offspring | |||
G | 113.209 Days | |||||
r | 0.024 Ind/ Days | |||||
Optimal Age for Sexual Maturity | 120 Days |
SEPTEMBER 2018 RESULTS:
Life Table | ||||||
Age class (days) | Sample/ age class | Number/ age class | Survival rate | Fecundity | Offspring/ individual | Age-weighted fecundity |
x | n(x) | n(x) | l(x) | b(x) | l(x)*b(x) | l(x)*b(x)*x |
0 | 9 | 150 | 1 | 0 | 0 | 0 |
30 | 12 | 141 | 0.94 | 0 | 0 | 0 |
60 | 26 | 129 | 0.86 | 0 | 0 | 0 |
90 | 29 | 103 | 0.687 | 24 | 16.48 | 1483.2 |
120 | 33 | 74 | 0.493 | 27 | 13.32 | 1598.4 |
150 | 18 | 41 | 0.273 | 29 | 7.93 | 1189 |
180 | 16 | 23 | 0.153 | 32 | 4.91 | 883.2 |
210 | 7 | 7 | 0.047 | 0 | 0 | 0 |
240 | 0 | 0 | 0 | 0 | 0 | 0 |
Sum | 150 | |||||
R0 | 42.63 | offspring | ||||
G | 120.89 | days | ||||
r | 0.03 | individuals/ day | ||||
Optimal age for sexual maturity | 120 | days |
Measurement LabReport
- Length Measurement
- What is the importance of the International System ofMeasurement?
- Based on lab, Section I, Exercise 1, how thick is a stack often dimes relative to a meter stick? Provide your answer incentimeters.
- What is the numeric relationship between millimeters,centimeters and decimeters?
- Metric Units
- The scientific notation for 2,600 is
- 500 centimeters equals metersor _ millimeters.
- 2080 meters equals _ kilometers or_ decimeters.
- Volume Measurement
- Draw and name three laboratory apparatus used to measurevolume. Sign, date and prepare an image of your drawing and includeit with this lab report.
- A laboratory procedure requires a measurement of 0.5 ml offluid to the nearest 1/10 ml. Which pipette should be used?
- Based on lab, Section III, Exercise 5 explain how to determinethe volume of a marble.
- Mass Measurement
- What is the difference between weight and mass?
- 50 Kilograms equals grams.
- Describe the procedure for determining the mass of a coin usinga triple beam balance.
- In the lab, Section IV, Exercise 7 determine the mass of abeaker and complete the table below. Here is a hint: the sum of themasses of the three beams equals the total mass of the beaker.
Middle beam mass | g | |
Back beam mass | g | |
Front beam mass | g | |
Total mass | g |
- Temperature Measurement
- Your friend’s temperature is 39.5°C. Does this indicate aproblem?
- Convert 20°C to °F.
- If you measured the temperature of a glass of ice water with aCelsius thermometer, what would you estimate the temperature tobe?
Summary Questions
- Record the basic unit of metric measurement of each of thefollowing:
Length | |
Volume | |
Mass | |
Temperature |
- Explain how the metric units for length, volume, and mass arerelated.
- What is the mass of 800 ml of water?
- Which metric units of length would most conveniently measurethe following:
Pen | |
Car | |
Ant | |
Dallas to San Diego |
- An object is 24 cm long. What is the length in mm?
- What size pipette accurately measures 0.8 ml of fluid? Indicatethe volume and accuracy of the pipette.
- In most countries, gasoline is measured in liters. The volumeof one liter is approximately one quart. There are four quarts inone gallon. In Europe, gasoline costs $1.86/liter. How does thisprice compare with the cost of gasoline in the United States?Please show your work.
- Convert the following numbers to scientific notation.
-0.00125 |
|
80,200.0 | |
7,231 |
|