BICH 410 Chapter Notes - Chapter 5: Radioimmunoassay, Peptide, Antibody

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10 Sep 2016
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Primary structure is the amino acid sequence of its polypeptide chain or chains if the protein consists of more than one polypeptide. Proteins are synthesized by stepwise polymerization of amino acids in orders speci ed for speci c amino acids. * most polypeptides contain 100-1000 residues with the average being. The size of polypeptides is indicative of the type of function that particular chain will carry out. Proteins that leave the cell must undergo some sort of puri cation process or else they will be rendered un t for the process they were made to do. To keep a protein stable the ph, temperature, presence of degradative enzymes (proteases & nucleases), adsorption to surfaces, and long-term storage must be adequate or inactive in the case of enzymes. Assay- way to quantitatively detect a puri ed substance. Coupled enzymatic reactions allow for two reactions to quantify the substance that is trying to be found.

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