BSCI 105 Chapter Notes - Chapter 20: Bacterial Artificial Chromosome, Recombinant Dna, Dna Ligase

68 views8 pages

Document Summary

Recombinant dna- dna molecules formed when segments of dna from two different sources are combined into one, which helps to analyze genes and gene expression. Biotechnology- manipulation of organisms to make useful products. Dna cloning yields multiple copies of a gene or other dna segment. Then, plasmid is returned to bacterial cell, making. Through divisions, clones are made and dna is passed recombinant bacteria onto descendants: useful- amplify gene and to produce a protein product. Gene cloning and genetic engineering rely on the enzymes that cut. They are joined together by dna ligase (catalyze covalent bonds that close sugar phosphate backbones of dna strand) Cloning a eukaryotic gene in a bacterial plasmid. Products include recombinant plasmids and many nonrecombiant plasmids suitable conditions, cells take up foreign dna by transformation: plate the bacteria on nutrient. Only cells with plasmids will reproduce in these conditions because they are only ones with amp gene resisting ampicillin.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers
Class+
$8 USD/m
Billed $96 USD annually
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
30 Verified Answers

Related Documents

Related Questions