Bio 97 PCR.docx

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Biological Sciences
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Rahul Warrior

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PCR PCR has revolutionized medicine, forensics, and experimental biology • Pre-implantation genetic diagnosis as an application of PCR • Forensics (the Innocence Project) • Screening blood products for diseases • Viral infections in wild monkeys by collecting feces • Sequencing the Neanderthal genome • 95°C→denatures DNA by breaking the hydrogen bonds between strands • 50-65°C→temperature is reduced to allow primer to bind (anneal) to DNA • 72°C→temperature is raised to the optimum for the thermostable polymerase (Taq) to extend t
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