Bio 97 Splicing.docx

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Department
Biological Sciences
Course
BIO SCI 97
Professor
Rahul Warrior
Semester
Fall

Description
mRNA Splicing • Occurs at sites determined by consensus sequences • Requires multiple proteins • Takes place in the nucleus • Most of the RNA is discarded in mammals • Splicing pattern can vary under different conditions (alternative splicing) • Adomain is an independent folding unit • In general, each domain of the protein is encoded by a different exon • This allows new proteins to be generated from the different domains/exons/parts Exons are modules that can be linked up in different ways The Exon shuffle model is like making up new creatures from old parts . . . Alternative splicing of the same transcript can give rise to different proteins Introns are usually degraded and the exons join together Need to know: splicing occurs and requires a consensus start and that splicing usually occurs in one direction only. Start at the beginning an
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