Mapping Intro.docx

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Biological Sciences
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Rahul Warrior

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Mapping Intro 1. Discovering the molecular identity of a gene 2. Creating genetically-identical individuals 3. Engineering and propagating new pieces of DNA • “Forward genetics”: start from a phenotype, identify the gene. Same sort of problem as with human pedigrees: what is causing the problem? • Different possible approaches for forward genetics: • gene mapping (today and next lecture): find gene position in the genome • cloning by complementation • Other ways to clone a gene: • cloning by hybridization • screening a library for specific activity applications to mapping • Alarge number (>10,000) of human diseas
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