PCB 3063 Chapter Notes - Chapter 13: Nucleoside Triphosphate, Consensus Sequence, Sigma Factor
23 Jul 2018
School
Course
Professor
Document Summary
Rna polymerase, required for transcription, recognizes ribonucleotide triphosphates but not deoxyribonucleotides. Sigma factor associates with the core enzyme to form a haloenzyme: 2. Haloenzyme binds to the -35 and -10 consensus sequences in the promoter, creating a closed complex: 3. Haloenzyme binds the promoter tightly and unwinds the dsdna, creating an open complex: 4. A nucleotide triphosphate complementary to the dna at the start serves as the first nucleotide in the rna molecule: 5. 2 phosphate groups are cleaved from each subsequent nucleotide triphosphate, (cid:272)reati(cid:374)g a(cid:374) rna (cid:374)u(cid:272)leotide that is added to the 3" e(cid:374)d of the growing rna molecule: 6. A rho-independent terminator contains an inverted repeat followed by a string of approximately six adenine nucleotides: 2. The inverted repeats are transcribed into rna: 3. The string of u/s causes the rna polymerase to pause: 4. The inverted repeats in rna fold into a hairpin loop: 5.
