LIFESCI 7C Chapter N/A: LIFESCI 7C Chapter N/: LIFESCI 7C Chapter N/: LIFESCI 7C Chapter : LS7C Week 5 Day 7 Reading
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The ability to determine the nucleotide sequence of dna molecules has given a tremendous boost to biological research. Whenever a dideoxynucleotide is incorporated into a growing daughter strand, there is no hydroxyl group to attack the incoming nucleotide, and strand growth is stopped dead in its tracks (fig. For this reason, a dideoxynucleotide is known as a chain terminator. dideoxynucleotide a nucleotide lacking both the 2 and 3 hydroxyl groups on the sugar ring. By including a small amount of each of the chain terminators in a reaction tube along with larger quantities of all four normal nucleotides, a dna primer, a dna template, and dna polymerase, Sanger was able to produce a series of interrupted daughter strands, each terminating at the site at which a dideoxynucleotide was incorporated. http://www. macmillanhighered. com/brainhoney/resource/6716/digital_first_content/trunk/test/morri s2e/asset/img_ch12/morris2e_ch12_fig_12_17. jpg. Sanger sequencing a procedure in which chemical termination of daughter strands help in determining the dna sequence.
