MCD BIO 165A Chapter Notes - Chapter clathrin: Triskelion, Clathrin, Bank Secrecy Act

• School: UCLA
• Department: Molecular, Cell, and Developmental Biology
• Course: MCD BIO 165A
• Professor: arispe

Document Summary

Formed by 3 heavy chains & 3 light chains. Once structure fulfills function, disorganize themselves & contribute to pool of individual molecules. Recycling of individual units as a recurring theme. Accessory proteins in clathrin coat formation: ap2. Adaptor molecule binding to receptors (binding to cargo) & triskelions. When activated, goes to cytosol, captures triskelions, go to cargo. Triskelions do not directly bind to receptors. Intermediary between receptor & clathrin: bar/f-bar proteins. Important in membrane deformation & vesicle budding off from donor membrane. Bar recruited by high level of pip2. Pip2 mediates bar binding -> membrane bending. Bar works with dynamin (gtpase) => vesicle budding off from donor compartment. Low pip2 level mediates bar disassembly & clathrin coat polymerization: summary 4 stages in clathrin organization. Cargo binding to receptor & ap2 recruitment -> initiate & facilitate clathrin polymerization. Invagination phase: requires bar, pip2 -> bend membrane. Maturation: triskelion polymerization concluding, neck forms & recruits dynamin &