MIMG 101 Chapter Notes - Chapter 2: 16S Ribosomal Rna, Archaea, Eukaryote

• School: UCLA
• Department: Microbiology, Immunology, and Molecular Genetics
• Course: MIMG 101
• Professor: G U N S A L U S& B R A D L E Y

Document Summary

Pathogen in all diseased not in the healthy. Have to cultivable - not viruses, parasites that need host to reproduce. Disease can manifest differently if patient is immunocompromised. Sticks to negatively charged molecules on both gram positive and negative. Gram neg dye pink (also dyes gram pos but does not show up) Electron state changed and releases wavelength in diff state. Use enzyme instead of ff to show color. When primary region of antibody w/ ff attaches directly to localized proteins in an organism. Fluorophore of an antibody binds to constant region of host. This allows better visualization - more binding of ff second antibodies to primary antibody. Secondary antibodies are more common/general than primary ones that are more specific to indivs. Using secondary - you don"t have to indiv label and generate yourself. Archae and bacteria are diff b/c of ether bonds in archae and ester bonds in bacteria. Presence/absence of histones - no bacteria have them.