MCDB 1A Chapter Notes - Chapter 13: Atp Hydrolysis, Okazaki Fragments, Chromosome
10 Oct 2016
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Circumstantial evidence: in the right place, present in right amount, varied among species. Dna from one type of bacterium genetically transforms another type. Some substance from the dead s pneumococcus cell could cause a heritable change in the affected r cells. Eliminating other possibilities: results repeated in all trials except when treated with dnase (destroys dna) Positive experiment: isolated virtually pure dna from a cell-free extract containing the transforming substance. Viral infection experiments confirmed that dna is the genetic material. Problem: dna made up of only four simple nucleotides, too uniform to be able to confer all the functions and variety. Aka they thought protein also helped factor into it. Solution: figure out what part of the virus - dna or protein - enters host cell to cause genetic change. Eukaryotic cells can also be genetically transformed by dna. Transfection: transformation of eukaryotic cells by dna.
