BIOS 220 Chapter Notes - Chapter 10: Ion, Molecular Cloning, Insulin
Document Summary
Dna fragment isolation and amplification for lab-work: fragmentation via restriction endonuclease enzyme that cuts dna at specific sequences (ecori). Dna polymerase is used for amplification of fragments. Dna technology: techniques for obtain, amplify, manipulate dna fragments. Genetic engineering: application of dna tech to biological, medical, agricultural problems. Genomics: extension of tech to global analysis of n. a s in nucleus, cell, organism, group of species. 10. 1 overview: isolating and amplifying specific dna fragments. Amplification: tricking dna replication machinery into replicating required segment in vivo or in vitro. In vivo: fragments of donor dna (w/sample of interest, often entire genome) are inserted into vectors (plasmid/bacterial virus that carries/amplifies gene). 1st: donor dna cut up by restriction endonucleases into 100s-1000s of smaller fragments. 2nd: fragments inserted into vector chromosome forming recombinant dna, which is transferred to bacterial cells which each take up one recomb. molecule. Dna cloning: amplification via dividing these cells, each w/the recomb.
