BIOL 221 Chapter Notes - Chapter 5: Rolling Circle Replication, Hfr Cell, Auxotrophy

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> suggests that physical contact between 2 strains needed for wt cells to form. > growing each strain individually in un-supplemented minimal medium didn"t work/ no prototrophs arose. > suggests recomb of genes had occured between genomes of 2 strains. > or instead of gene exchange, they leak substances that other cells can absorb to grow. William hayes (1953) found that conjugating parents acted unequally (one parent would transfer some/ all of genomes into other cell) -> donor & recipient. Transfer of genetic material in conjugation is not reciprocal (one way transfer) Variant of original donor strain would not produce recombinants on crossing with recipient strain/ donor lost ability to transfer genetic material & changed into recipient type strain. Donor could regain donor ability by associating with other donor strains/ transmitted rapidly between strains during conjugation. Strains with f can donate (f+) are donors, strains that don"t have f and cannot donate are recipients (f-)

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