BIOL 2100 Chapter Notes - Chapter 20.1: Molecular Cloning, Recombinant Dna, Direct Manipulation Interface
28 Nov 2016
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20. 1 dna sequencing and dna cloning are valuable tools for genetic engineering and biological inquiry. Biotechnology manipulation of organisms to make useful products. Nucleic acid hybridization base pairing of one strand of nucleic acid to complementary sequence on strand from another nucleic acid molecule. Genetic engineering direct manipulation of genes for practical purposes. Dna sequencing dna cut into fragments then sequenced: method of dideoxy chain termination sequencing. 1 dna strand used to as template for synthesis of complementary fragment: sequencing by synthesis. Specific strand in each strand immobilized and complementary strand made: third generation sequencing. Involves moving long dna strand through a pore, detecting bases by interruption of electrical current. Making multiple copies of a gene or other dna segment: dna cloning. Uses bacteria and their plasmids (circular bacterial dna) Dna (gene of interest) inserted into plasmid. Bacteria with recombinant dna reproduces and forms clones of cells.
