BIOL 2100 Chapter Notes - Chapter 20: Polymerase Chain Reaction, Reverse Transcriptase, Dna Microarray

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20. 2 biologists use dna technology to study gene expression. Analyzing gene expression: studying expression of single genes. We can detect mrna by nucleic acid hybridization with molecules of complementary sequence (which is called nucleic acid probe) Cloned gene used as template and complementary probe made. Solution with probe molecules applied to observed embryos, and probes hy(cid:271)(cid:396)idize with (cid:272)o(cid:373)ple(cid:373)e(cid:374)ta(cid:396)y se(cid:395)ue(cid:374)(cid:272)es o(cid:374) (cid:373)rna"s. Reverse transcriptase polymerase chain reaction - turns mrna into double stranded dna. Enzyme reverse transcriptase used to make reverse transcript of each mrna molecule. Short complementary strand of thymines (complementary to poly-a tail) acts as primer. After degradation of mrna, second strand made by dna polymerase. Resulting dna called complementary dna (cdna: studing the expression of interacting groups of genes. Systems approach studying the expression of large groups of genes. Tiny amounts of a large number of single-stranded dna fragments representing different genes fixed to a glass slide in a grid.

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