BIOL 1105 Chapter Notes - Chapter 14: Dna Polymerase Iii Holoenzyme, Dna Gyrase, The Double Helix
8 Oct 2018
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Prokaryotic replication: coli contains a single circular molecule of dna (chromosome) Something to copy- the template, two individual stands of the parent molecule. Something to do the copying- enzyme called dna polymerase, incorporates in nucleotide at a time into a dna strand. Building blocks- individual nucleotides to be assembled into new. Dna stand (6,000 finish notes off class slides. The e. coli chromosome contains a single replicon. A region of dna controlled by an origin. All 3 synthesize dna in the 5" to 3" direction. All 3 have 3"-to-5" exonuclease activity (proofreading: rna polymerase makes the primer, dna polymerase extends the primer. The double helix must be unwound to allow replication. Helicases use energy from atp to unwind dna. Single-strand-binding proteins (ssbs) coat strands to keep them apart. Dna is composed of two antiparallel stands: 5"-3" and 3"-5" Dna polymerase can only synthesize in 5" to 3" direction.
