L41 BIOL 4810 Chapter Notes - Chapter 6: Ethanol Precipitation, Lysis, Centrifugation
12 May 2020
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Scenario 1 genomic dna: goals, separate hmw (high molecular weight) dna from, lipids, rna (separating rna from dna, because we don"t want dna, protein (lyse open cells lots of proteins in cells break interactions with. Purification: the goal: be able to study a single species in a test tube, with other cellular components removed. It is important to understand these techniques in order to appreciate the significance and limitations of purifications, and biochemical studies more broadly: purification is a fractionation process. By knowing a bit about the properties of the protein, these properties can be used to isolate the protein of interest from the cellular milieu: liquid-liquid extraction = fractionation. Proteins are biological polymers that fold into complex: reminder to keep the structure working. Need to isolate a single protein of interest: searching for a needle in a haystack, step: one: make the needle (or protein of interest) a much more substantial portion of the mixture .
