BIOL 461 Lecture Notes - Lecture 6: Gap Gene, Polytene Chromosome, Pattern Formation
12 Feb 2018
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Biol 461 lecture 6 notes (stop at slide 63) 26/01/2018. Mutants had clean phenotypes, and caused changes in the embryo that really suggested/showed the existence of gene functions that are involved in establishing very specific aspects of the pattern in embryogenesis. Able to group the mutant by common phenotypes, then complementation tests: making trans heterozygotes, putting one mutant over the other. Top is the banding pattern of the polytene chromosomes cytological map. Shaded in rectangles = deletions if the deletio(cid:374) does(cid:374)"t (cid:272)o(cid:373)ple(cid:373)e(cid:374)t the (cid:373)utatio(cid:374) (cid:373)ea(cid:374)s the gene is somewhere that is taken out by that deletion. If these t(cid:449)o deletio(cid:374)s do(cid:374)"t (cid:272)o(cid:373)pli(cid:373)e(cid:374)t then you know that your gene is in there somewhere. Pair rule genes: mutants showed pattern deletions in every other segment. Gap gene mutants ( giant, hunchback, knirps, kr uppel, and tailless) Homeotic genes (transform one body part to another body part) They found mutants that had very specific clean alterations of the body pattern.
