BIOL 200 Lecture Notes - Lecture 50: Atp Hydrolysis, Helicase, Base Pair
Document Summary
Developmental timing mutants in c. elegans helped to elucidate a new pathway (cid:120) (cid:120) (cid:120) Lin-4 encodes a small rna that has considerable homology (antisense) to regions of the lin-1(cid:1008) (cid:1007)". Utr: 60 nucleotides (lin-14) that could be cut down to 20 nucleotides (lin-4) Lin-4 rna is synthesized as a longer precursor then it has to be processed, after which it binds its target sites and affects translation of the lin- 14 mrna (cid:120) argonaute, which is a component of risc, and. Dicer are both involved in this pathway: they both recognize the substrates and chop them down, but the outcomes are different. Rnai pathway, but may affect stability by enhancing deadenylation or, it may block its translational efficiency. For lin-4/lin-14, this occurs at the level of translational elongation. Rnai vs microrna (cid:120) both pathways are triggered by a dsrna molecule (cid:120) sirnas base pair with 100% complementarity and wipe out rnas.
