BIOLOGY 1A03 Lecture Notes - Lecture 3: Transcription Bubble, Stem-Loop, Consensus Sequence

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BIOLOGY 1A03 Full Course Notes

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Mo d u le 1 tra n s c rip tio n. Un it 1: re a d in g th e blu e p rin t: dna acts as a blueprint, central dogma: proteins provide structure and carry out many essential activities in a cell. Un it 2: tra n s c rip tio n in p ro ka ryo te s: dna is transcribed from a template strand, transcription has a start and end site. Transcription is initiated at a promoter sequence and ends at a terminator sequence. transcription is synthesized in a 5"-to-3" direction. Both dna strands serve as templates for transcription. For rna to be transcribed, rna polymerase (enzyme) attaches to specific promoter regions of dna. Rna polymerase will recognize and bind to a promoter, and once transcription is initiated it will move along dna in a 3"-to-5" direction synthesizing an rna molecule from 5" end to 3" end: transcription initiation.

Related Questions

1) In Eukaryotes, repetitive sequence inclues:
A) Centromere sequences
B) Telomere sequences
C) All of the above

2) Satelit DNA:
A) Was orignally discoverd in E. Coli
B) Represents plasmid DNA sequences
C) Contains repetitive DNA sequences
D) Has the same density as main band DNA
E) All of the above

3) Inversions _____ The number of crossover offspring.
A) Decrease
B) Increase
C) Do not change
(I Believe the answer is "C" ^. can you explain why?)

4) In human genome, repetitive DNA is estimated to be ___ of the total genome.
A) .1%
B) 1%
C) 10%
D) 50%
E) 90%

5) In E. Coli, the promoter sequence:
A) Contains a short AT rich sequence
B) Binds transcription factors
C) All of the above

6) In eukaryotic transcription:
A) Transcription factors bind to the promoter and then RNA polymerase binds.
B) Transcription factors bind to RNA polymerase which then binds to the promoter
C) Transcription factors are NOT required to initiate transcription

Explanation for each would help immensely. THANK YOU in advance for your time!!!

cerisebadger105

During transcription in eukaryotes, a type of RNA polymerase called RNA polymerase II moves along the template strand of the DNA in the 3'â5' direction. However, for any given gene, either strand of the double-stranded DNA may function as the template strand. Which of the following initially determines which DNA strand is the template strand, and therefore in which direction RNA polymerase II moves along the DNA?

A. the location of specific proteins (transcription factors) that bind to the DNA

B. the specific sequence of bases along the DNA strands

C. the location along the chromosome where the double-stranded DNA unwinds

D. the position of the geneâs promoter on the chromosome

E. which of the two strands of DNA carries the RNA primer

5. Which of the following statements regarding eukaryotic transcription is FALSE?

a. RNA polymerase II has a critical carboxy-terminal domain that is phosphorylated during

active transcription.

b. General transcription factors are designated TFII followed by a number, and they function in initiation of transcription across the eukaryotic genome.

c. The most highly transcribed eukaryotic genes contain CpG islands upstream of their transcription start site rather than TATA boxes.

d. Distal enhancers, like promoter-proximal elements, are composed of several small, 6â10 bp functional sequence elements.

BIOLOGY 1A03 Lecture Notes - Lecture 3: Transcription Bubble, Stem-Loop, Consensus Sequence

BIOLOGY 1A03 Full Course Notes

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