BIOLOGY 1A03 Lecture Notes - Lecture 3: Dideoxynucleotide, Reading Frame, Spectrogram

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Rosa Da Silva

Document Summary

Key components in in vitro replication (1985: karly mullis developed the ability to amplify dna in tube via the polymerase chain. Denaturation: double helix is unwound by the high temperature of the reaction mixture. Annealing: thermocycler will cool the solution to allow annealing of two primers (on opposite strands at each end of the target sequence) to their complementary sequences on the dna template on either side of the dna sequence of interest. Extension: taq polymerase extends/polymerizes the daughter strands using four dntps starting from the primers and moving in a 5"-3" direction. Each complete cycle results in two helices containing the desired target sequence portion of the template dna. Whole genome sequencing (1975: method developed by frederick sanger which is able to determine the sequence of a. Dna molecule: limitation was that it could only determine the sequence of small dna fragments.