BIOLOGY 2B03 Lecture Notes - Lecture 3: Histone H3, Ultracentrifuge, Centrifugation
1 Sep 2020
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Document Summary
Determination of amino acid sequence to predict sequence of encoding gene. Development of antibodies specific to a protein that can be used in immunofluorescence or in a. Method of detecting for the presence of a specific protein and estimating the concentration of the protein. Must be specific to the protein being studied and based upon its unique characteristics. If protein is an enzyme, then measure enzymatic activity by looking for release of product or consumption of substrate. If antibody unique to your protein exists, it can be used to monitor the presence of concentration of your protein (immunological antibody assay) Easily obtainable in large amounts (tissue, cultured cells, overexpression in another system) Contains high concentration of the protein of interest. Choose a cell type that is low in proteins that may co-purify. Co-purify = purify together due to similar characteristics to protein of interest. Low in proteases that may destroy the protein of interest.
