BIOLOGY 2B03 Lecture Notes - Lecture 3: Cytosol, Transfer Rna, Cell Membrane
20 Jan 2016
School
Department
Course
Professor
Document Summary
Ways to purify protein: structural analysis, determine the aa sequence, functional analysis or develop antibodies to a protein: develop a good assay, select a protein source, break open cells protein extract, solubilize protein. 11: differential centrifugation, separate based on mass and density, tube of crude cell extract: centrifuge at 1000g, transfer supernatant to fresh tube, centrifuge supernatant at 104g; repeat, centrifuge supernatant at 105g. To extract the vely charged proteins, it has to be washed with salt wash solution, warm wash solution or change ph of wash solution, or have vely charged beads inside column. Small beads can be extracted via wash or spinning at low speeds. 38: limit of resolution, d, d the minimum distance between two objects that can be distinguished from one another. D better resolution: light microscopy, ex. Fluorophore is excited by uv lights an emits a fluorescent light which can be detected.
