BIOLOGY 2B03 Lecture Notes - Lecture 11: Terminal Deoxynucleotidyl Transferase, Tunel Assay, Substantia Nigra
27 Apr 2016
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Cells are always added to our body by mitosis and taken away by apoptosis. Network of proteins is required to signal and regulate apoptosis. Week 6: skin forms webbing between digits on hands and feed. Week 11: webbing disappears and digits are resolved; without cell death, webbing remains. Detects nicks in the dna of apoptotic cells. Experiment: mouse embryos were stained with a dye so cells undergoing apoptosis are bright green. Tunel assay takes advantage that dna in apoptosis contains dna breaks or nicks dutp is added into the nicks by using the enzyme terminal deoxynucleotidyl transferase: fluorescently labelled dutp allows for the detection seen via bright green. Tadpoles undergo cellular apoptosis to lose the tail. Apoptosis in the tail is stimulated by increase in thyroid hormone in blood, which is also seen in tail loss during human embryonic development.
