BIOL 311 Lecture Notes - Lecture 21: Missense Mutation, Agarose Gel Electrophoresis, Wobble Base Pair
Document Summary
Polymorphisms in region 2 (coding region) resulting in mutations. Change usually results in one or more codons in the mrna being changed. The code for which a codon codes is said to be degenerate (more than one codon coding for the same amino acid) Change in this position is least likely to cause a change in the amino acid being coded for. Rna polymerase bings at promoter to begin transcription (+1 site), and binds at terminator to end transcription. Band intensity on agarose gel represents concentration of a certain allele. Snps and sslps can become mutations in the coding region, however, they will result in different types of changes in the protein. Four different types of changes (mutations) can result from snps. Coding strand: same sequence as mrna, however, thymine (t) is replaced with uracil (u) Results in a nucleotide change (snp) which does not result in a change in amino acid, thus, protein produced will be the same.
