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BIOL 331 Lecture Notes - Lecture 14: Rer A, Copi, Copii

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rosefrog223
10 Feb 2018
School
University of Calgary
Department
Biology
Course
BIOL 331
Professor
Vanina Zaremberg

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Document Summary

Depends on the formation of a vesicles called a copi- coated vesicle. Proteins may be synthesized in golgi that now can"t. Golgi resident enzymes in a trans-to-cis direction. Coat is made up of a complex called coatamer that contains 7 proteins. One of the proteins is called arf1, which is another g- protein, and has a similar function to sar1 in anterograde transport. Proteins that are destined to remain in er have the sequence kdel, which is recognized by the kdel receptor (imp1) and functions as an address tag. This sequence is recognized by the kdel receptor, which looks for proteins with the. Kdel sequence to help carry proteins in the. Kdel receptor membrane proteins that act as receptors for binding biomolecules to be transported. Coatamer (one of which is arf1) membrane proteins that allow for budding and vesicle formation. Microtubules and motor proteins means through which vesicle can find its way to proper compartment.

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Related Questions

Materials traffic through the endomembrane system of eukaryotic cells via vesicles. There are two coat proteins COP I and COP II that assemble vesicles for transport along microtubules. COP I coated vesicles undergo retrograde transport, for example, to move materials from the Golgi back to the ER. COP II coated vesicles undergo anterograde transport to move materials from the ER outward.

(1 pt) Which motor protein (myosin, kinesin or dynein) interacts with COP I vesicles? ______________

(1 pt) Which motor protein interacts with COP II vesicles? ______________

(1 pt) Some proteins that traffic in this system contain a KDEL amino acid signal sequence for retention in the ER that interacts with a KDEL receptor protein. Would you expect the KDEL receptor protein to be found associated with COP I or COP II coated vesicles? _________

1) how does the timing and location of Arf1-GTP hydrolysis contribute the transport of COPI coated vesicles?

2) Consider cargo proteins that are destined for secretion to the extracellular space. For each of the following pairs of mutations or conditions, predict where the cargo will end up (ex. in the cytoplasm, a specific organelle, transport vesicles, multiple places, extracellular space) for: (i) & (ii) Each individual condition; & (iii) The combination of both.

A.(i) Normal Sar1 is mutated so that it hydrolyzes GTP immediately after binding GTP.

(ii)The signal recognition particle is mutated to be unable to bind to the SRP receptor.

(iii)Both


B.(i) A mitochondrial signal sequence is added to the C-terminus of the cargo protein.


(ii)v-SNAREs on COPII coated vesicles are mutated to be unable to bind t-SNAREs


(iii)Both


C. (i) COPII coat proteins are mutated so they don’t bind to the ER membrane.


(ii)COPI coat proteins are mutated so they don’t bind to the Golgi membrane.


(iii)Both


D.(i) Normal Sar1 is mutated so it binds tightly to GDP and cannot exchange it for GTP. (This leads to Sar1*-GDP being the only form of the protein in the cell.)

(ii) A transmembrane domain is added to the cargo protein.

(iii)Both

1) In the major biosynthetic pathway, what is the order in which mRNA moves through the various organelles?

2) Compare and contrast the structure and function of the smooth endoplasmic reticulum and the rough endoplasmic reticulum?

3) Is SAR1 COPI or COPII? What type of protein is this? What causes SAR1 to active and what protein is necessary for this to occur?
4) Dynamin binds where clathrin meets the plasma membrane. As dynamin binds, it forms a ring that is then pinched so that the vesicle can be released from the plasma membrane. What allows for this to occur?

5) What are three different mechanisms of protein translocation? Briefly describe the state of the proteins when translocated in each of these ways.

6) What is the KDEL sequence stand for and how does it function within the cell? What type of transport is it associated with?

7) In what cellular compartment would a glycoprotein be expected to have its greatest mannose content? Its greatest N-acetylglucosamine content? Its greatest sialic acid content?

8) Would you expect the properties of the cisternal side of Golgi membranes to be more similar to the extracellular or cytosolic side of the plasma membrane? Why?

9) ________ are the site of cellular digestion, in which digestion occurs with the help of 50 __________. _____ digest proteins and ____ digest lipids.

10) Name two proteins you would expect to find as integral components of the RER membrane that would be absent from the SER? Name two proteins of the SER that would not be present in the RER?