BIOB10Y3 Lecture Notes - Lecture 12: Scanning Electron Microscope, Förster Resonance Energy Transfer, Atomic-Force Microscopy
29 Aug 2016
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Chapter 18 techniques in cell and molecular biology. Condenser lens gathers diffuse rays from light sources & illuminates the specimen. The light rays focused on the specimen by the condenser lens are collected by the microscopes objective lens. The ocular lens forms an enlarged & virtual image. A third lens system located in the front part of the eye uses the virtual image produced by the ocular lens as an object to produce a real image on the retina. When the focusing knob of the light microscope is turned, the distance b/w the specimen & the objective lens changes. The resolution attained by a microscope is limited by diffraction. The denominator of the equation is called the numerical aperture (n. a. ) Constant for each lens, a measure of its light-gathering qualities. The max useful magnification of a light microscope ranges b/w 500 & 1000x the numerical aperture of the objective lens being used.
