MICR 302 Lecture Notes - Lecture 9: Lac Operon, Antibody, Auxotrophy

• School: University of Victoria
• Department: Microbiology
• Course: MICR 302
• Professor: Briant

Document Summary

Effects of the stringent response: (referring to mechanism on slide 40) Can have effects on transcription and translation. Alarmone can inhibit replication down down regulating translation in response to starvation. Effects on transcription can be either direct or indirect (through sigma factor) Consider: direct binding to rnap, influence on promoter binding, influence on sigma choice, effects with dksa, sigma1. 2 - discriminator interaction. Note: these all effect however they are categorized into two separate processes (study: slide 42) > change in sigma factor preference without a change in relative sigma factor concentrations. Alarmone destabilizes sigma70:rnap association which contributes to alternate factors being more likely to be bound to rnap (alters which sigma factor binds without altering the amounts of alt sigma factors) Alters which regions are expressed at any given time. First way in which we can direct the polymerase to different genes. This needs dksa (binds in secondary channels where the nucleotides come into the rnap)