Biochemistry 2280A Lecture Notes - Lecture 18: Tata-Binding Protein, Chromatin Remodeling, Tata Box

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15 Jan 2017

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6 difference in transcription between prokaryotes and eukaryotes: there are 3 types of rna polymerases in eukaryotes where prokaryotes only have 1. Polymerase iii: trnas, ss rrnas: there are no operons in eukaryotes. Each gene is transcribed as a single unit. Every gene has its own promoter, every gene makes 1 protein. Do not have -10 and -35 sequences or sigma factor. Promoter recognition is determined by a set of proteins. One of which recognizes a tata element/box (which is found upstream from the start of transcription) Has a similar sequence, but is not a recognition site for sigma. Which in turns recruits other proteins (tfiia, tfiib, etc) 12 go look: in eukaryotic regulatory proteins often bind dna several thousand bases pairs from the start site. Prokaryotic regulatory elements are up to a few hundred base pairs away (much closer) Dna is flexible - can move in between! (dna looping) Bring elements close together: combination control.

Related Questions

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

What are the three processes in post-transcriptional processes that must be completed before transcripts can be translated in eukaryotes?

A.	Binding of snRNPs, addition of a poly A tail, splicing of introns
B.	Binding of snRNPs, transporting, synthesizing of ribose
C.	Capping, transporting, synthesizing of ribose
D.	Binding of snRNPs, capping, splicing
E.	Capping, splicing, addition of a poly A tail

Which of the following key differences between prokaryotic and eukaryotic transcription are TRUE? Check all that apply.

	A. prokaryotic transcription occurs in the cytoplasm, while eukaryotic transcription occurs in the nucleus
	B. eukaryotic transcription is carried out by 1 RNA polymerase, while prokaryotic transcription is carried out by 3 different RNA polymerases
	C. eukaryotic transcripts are processed, while prokaryotic transcripts are translated while transcription is still occurring
	D. prokaryotes have sigma factors for specificity of binding whereas eukaryotes have multiple general transcription factors
	E. prokaryotes have to worry about unpackaging the DNA for transcription, while eukaryotes have almost naked DNA not bound up by nucleosome

whitehippopotamus545

Which type of RNA is unique to eukaryotes?

		mRNA
		rRNA
		tRNA
		snRNA
		Its a trick, all cells have all four types of RNA.

1 points

QUESTION 2

The region of DNA to which RNA polymerase binds to initiate transcription is the:

		origin
		promoter
		enhancer
		none of the above are correct

1 points

QUESTION 3

Holoenzyme:

		is RNA polymerase core enzyme.
		is sigma factor.
		is core plus sigma factor.
		none of the above are correct.

1 points

QUESTION 4

Which of these is formed FIRST when RNA polymerase binds to a promoter?

		The closed promoter complex
		The open promoter complex
		A rho-independent terminator
		none of the above are correct.

1 points

QUESTION 5

A hairpin loop followed by a run of Us:

		is the sequence formed in the sense strand of a gene using a rho-independent terminator.
		is the sequence recognized by the rho protein.
		is the structure formed in RNA that causes rho-independent termination.
		is formed immediately after the open promoter complex is formed.

1 points

QUESTION 6

RNA polymerase:

		reads the template strand of the DNA in the 3' to 5' direction.
		synthesizes the RNA in the 5' to 3' direction.
		can begin transcription without a primer.
		all of the above are correct.

1 points

QUESTION 7

Which of the following statements is FALSE?

		Bacteria like E. coli have only one type of sigma factor.
		RNA polymerase has no proofreading activity.
		The promoter of a gene is upstream of the RNA-coding region.
		Bacterial transcriptional terminators are copied into RNA to cause termination.

1 points

QUESTION 8

If the template strand of a gene has the sequence 5' ATGCGAT 3', then:

		the RNA produced will have the sequence 5' AUGCGAU 3'.
		the RNA produced will have the sequence 5' UAGCGUA 3'.
		the RNA produced will have the sequence 5' AUCGCAU 3'.
		None of the above are correct.

1 points

QUESTION 9

The sense strand:

		is the lagging strand
		is also the template strand.
		always goes left to right.
		none of the above are correct.

1 points

QUESTION 10

Which of these is transcribed but not translated?

		mRNA
		tRNA
		rRNA
		all of the above
		tRNA and rRNA are transcribed but not translated.

please help i am a bit confused

Biochemistry 2280A Lecture Notes - Lecture 18: Tata-Binding Protein, Chromatin Remodeling, Tata Box

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