Biochemistry 2280A Lecture Notes - Lecture 18: Eukaryote, Lac Operon, Nucleosome

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22 Nov 2017

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6 differences in transcription between prokaryotes and eukaryotes: three rna polymerases in euk, only 1 in pro, pol i- most rrna genes, pol ii- mrnas, pol iii- trnas, 5s rrna. Tata-element: similar sequence to -10 but not the same. Look at fig 7-12: in euk, regulatory protein soften bind dna several thousand base pairs from the start site, very big, eukaryotes can have factors bind near the start site or far away from the start site. Dna looping: dna can loop- a factor that is far away from the start site can loop out and can bind to site, combinational control, groups of proteins work together to determine the expression of a gene, ex. Lac operon: many factors regulating expression of a gene- some +ve, some -ve. Nucleosome positioning is influenced by many proteins (fig 8-11: chromain remodelling complex, histone acetylase, both can open up gene, doesn"t occur in prokaryotes since no chromatin.

Related Questions

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

Biochemistry 2280A Lecture Notes - Lecture 18: Eukaryote, Lac Operon, Nucleosome

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