Biology 2382B Lecture Notes - Lecture 1: Sox2, Apoptosis, Telomere

72 views7 pages

Document Summary

A cell functions to secure di erent enzymes. Replicate dna, transcribe rna, translate, sort proteins, secrete proteins. We aim to understand how macromolecules and organelles work together to allow cell function in tissues, and independently. By hypothesis driven method ask how something is working. Need to isolate the cell and let it grow in vitro (in a glass tube) We use cell cultures technique used to grow cells/ tissues outside the organism, in controlled conditions. Cells either adhere to matrix via adhesion to proteins in intracellular environment, or adhere to each other due to cell surface proteins. Cells are rst isolated from cell-cell interactions, or cell-matrix interactions, by: Mechanical fragmentation (grind up the entire tissue, and hope at least 1 cell remains intact and can be isolated) Trypsin (a protease enzyme that degrades protein, can cleave cell- matrix/ cell-cell interactions. But must not leave it there for too long)

Get access

Grade+
$40 USD/m
Billed monthly
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
10 Verified Answers
Class+
$30 USD/m
Billed monthly
Class+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
7 Verified Answers

Related Documents

Related Questions