Biology 2382B Study Guide - Midterm Guide: Pinocytosis, Hat Medium, Phospholipid

• School: Western University
• Department: Biology
• Course: Biology 2382B
• Professor: Robert Cumming

Document Summary

Ultimate goal: to understand how macromolecular systems and organelles work and cooperate to enable cells to function autonomously and in tissues. In vitro means to have cells outside of the body in an artificial manner. Cell culture is the technique used to grow cells or tissues outside the organism under strictly controlled conditions. Cells are isolated from any tissue by breaking down the cell-cell and cell-matrix interactions (use mechanical fragmentation, trypsin, edta). Cells taken from an organism or tissue stick together by sticky proteins. Can mechanically break them into smaller clumps with a filter. Then use enzymatic processes (such as trypsin) to break the sticky protein interactions: or we could use edtas - calcium and magnesium bind to sticky type proteins. Edta su(cid:272)ks up (cid:272)al(cid:272)iu(cid:373) io(cid:374)s so sti(cid:272)k(cid:455) p(cid:396)otei(cid:374)s do(cid:374)"t fold p(cid:396)ope(cid:396)l(cid:455). Cells are supplied with proper nutrients (amino acids, minerals, vitamins, salts, glucose, etc.