Biology 1002B Lecture Notes - Lecture 8: Electron Acceptor, Cellular Respiration, Mitochondrion
20 Mar 2016
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Use a syringe to introduce a compound, without air contaminaion. 0 to 2 minutes: no mitochondria, slope of 0, constant amount of oxygen. 2 to 4 minutes: mitochondria added to bufer, slope of 0, no change because there is no substrate. 4 to 6 minutes: nadh added, negaive slope, oxygen is consumed since it is the terminal electron acceptor in the etc. 6 to 8 minutes: adp + pi added, steeper negaive slope, ph gradient is reduced by adp and pi. Respiratory control: prevents the excessive oxidaion of nadh and fadh2. 8 to 10 minutes if adp and pi are not present: 2,4dinitrophenol (ripped freak, chemical uncoupler, even steeper negaive slope, there is no ph gradient. Mitochondria are no diferent than those in an animal cell. Make their own food using light and co2. G3p is exported from the chloroplast (3 turns of calvin cycle for 1 molecule)
