Biology 1002B Lecture Notes - Lecture 8: Acetyl-Coa, Glyceraldehyde 3-Phosphate, Cytosol
31 May 2016
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Measure respiration by looking at change of oxygen concentration. Oxygen electrode chamber: add chlamy cells, purify intact mitochondria, incubate in a buffer. Add oxygen via a syringe into solution and see the effect. Measuring the rate of cellular respiration: oxygen vs time. Adding mitochondria slope does not change. No change, no nadh, fadh to react. Adding adp + pi slope goes down a lot. Without the adp and pi the gradient is too big and etc can"t work properly: respiratory control. Adding ripped freak (pokes holes) slope goes down a lot more. No pressure on pumping protons, no ph gradient. Can flow through chain super fast, using a lot of oxygen. Chlamydomonas: autotrophic growth: doesn"t need to eat other things (use light) Co2 into calvin cycle produces adp+pi from atp and g3p: chloroplast in chlamy don"t export atp, export g3p. G3p turns into glucose or pyruvate in cytosol.
