CHEN 3701 Lecture Notes - Lecture 8: Pilot Experiment, Reaction Rate Constant, Reaction Rate
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Problem 1: in a pilot experiment, you add 10 nm enzyme to 10 m substrate and observe an initial reaction rate of 2. 0. You also perform binding experiments ato determine the association rate constant (1*106 m-1s-1) and dissociation rate constant (250 s-1). Write a material balance on the enzyme (not useful to do it on substrate) Plug in --> (ii) quasi-steady state assumption material balance on enzyme stays the same. Plug in #s: sketch a plot of initial reaction rate vs. substrate concentration for this system. I"m getting k = 380 m double kcat. Quasi steady state double kcat --> double vmax, km increases vmax doubles for both situations. Problem 2: consider an enzymatic mechanism in which product formation is reversible; i. e. enzyme can catalyze conversion of "product" to substrate: derive an expression for the rate of product formation using a pseudo-steady state approximation (pssa)