BIOLOGY 2EE3 Lecture Notes - Lecture 4: Sinorhizobium Meliloti, Transmission Electron Microscopy, Scanning Electron Microscope
15 Jun 2022
School
Department
Course
Professor
Document Summary
Topics to cover: morphology of bacterial cells, the cytoplasm, the bacterial cytoskeleton, the cell envelope, the bacterial cell surfaces, bacterial taxonomy. Studies primarily performed on proteobacteria and some firmicutes --- other phylogenetic distinct groups now being studied. Many model microbes are from this group: e. g. escherichia coli, bacillus subtilus, pseudomonas aeruginosa, caulobacter cresentus, Doubling time or generation time: time it takes to reproduce: generation time varies depending on nutrient availability, temperature, Transmission electron microscope: beam of electron generated from heat, cells are cross sections embedded in plastic like resin and stained with heavy metals, electrons are passes under vacuum. Scanning electron microscope: do not pass-through microbes, but reflect and detected by detector in an imaging computation, looks at the surface of the cells. Samples were fixed in 2% (v/v) glutaraldehyde and post- stained with uranyl acetate and lead citrate. Random sausage shape scattered around: cross section can be hotdog or hamburger cut.
