MCDB 3135 Study Guide - Quiz Guide: Northern Blot, Glycosidic Bond, Phosphodiester Bond

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Recognize the 5 bases and draw them, know which ones are purines and pyrimidines. Number the carbons in pentose (don"t forget ) and c/n in bases. Know which n in base attach to which c in pentose. Be able to recognize the 5" -> 3" orientation. Name hydrogen bonds between nucleotides, know they are not covalent bonds. Know that nucleosides tri-phosphate are precursors of dna synthesis. Show the minor and major grooves on dna. Be able to recognize the right-handed from a left-handed double helix. Recognize a and b types of helices. Know that a and b are both right-handed, a-type is typical rna, b-type typical dna. Identify whether a sequence can form a hairpin with or without loop. Recognize ribo- vs deoxy-ribonucleotides and be able to explain the difference: Know the consequences of this hydroxyl group: Be able to list the structural characteristics of rna:

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