Problem 7. A patient with Li-Fraumeni syndrome has a tumor with a mutation on only one allele of p53. Explain why this is not an example of loss of heterozygosity.(8pts)
Problem 8. A tumor virus produces a protein which inhibits p53. Is this viral protein an oncogene or a tumor suppressor? Why? (10pts)
Problem 9. Why might a tumor with mutations in p53 be resistant to radiation therapy? (8pts)
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Problem 10. Create a molecular diagram using this information: Double stranded DNA breaks activate ATM. ATM phosphorylates p53. When p53 is phosphorylated, it can travel to the nucleus and act as a transcription factor-to increase the expression of a protein called BAX. BAX is a protein that turns on apoptosis. Your answer should contain the components of this pathway (e.g. double stranded breaks, ATM, p53, BAX, Apoptosis, Cancer) and activate or inhibit signs (you can use words if you dont want to draw symbols). (16pts)
Problem 7. A patient with Li-Fraumeni syndrome has a tumor with a mutation on only one allele of p53. Explain why this is not an example of loss of heterozygosity.(8pts)
Problem 8. A tumor virus produces a protein which inhibits p53. Is this viral protein an oncogene or a tumor suppressor? Why? (10pts)
Problem 9. Why might a tumor with mutations in p53 be resistant to radiation therapy? (8pts)
2
Problem 10. Create a molecular diagram using this information: Double stranded DNA breaks activate ATM. ATM phosphorylates p53. When p53 is phosphorylated, it can travel to the nucleus and act as a transcription factor-to increase the expression of a protein called BAX. BAX is a protein that turns on apoptosis. Your answer should contain the components of this pathway (e.g. double stranded breaks, ATM, p53, BAX, Apoptosis, Cancer) and activate or inhibit signs (you can use words if you dont want to draw symbols). (16pts)
