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You can’t see phages with a standard light microscope. What is the resolution of an electron microscope compared with a light microscope? What is the reason for this increased resolution?

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Keith Leannon
Keith LeannonLv2
28 Sep 2019

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Related questions

26. Which of the following is the best microscope to use to visualize a cross section of skin
stained so that the cells are purple and the extracellular matrix is pink:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
27. Which of the following is the best microscope to use in order to visualize cell to cell
junctions at high magnification:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
28. A student is studying the tensile strength of a new metallic alloy. He wants to visualize
the fracture surfaces following tensile testing. Which is the best microscope for this
application:
a. Light microscope
b. Confocal microscope
c. Transmission electron microscope
d. Scanning electron microscope
e. Fluorescence microscope
29. A graduate student is studying the degradation properties of a new polymer that she
developed. She adds a fluorescent molecule to the polymer backbone and makes a thin
layer of the polymer on a glass slide. In order to visualize the polymer and measure its
degradation/disappearance over time, which is the best microscope to use:
a. Light microscope
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c. Transmission electron microscope
d. Scanning electron microscope
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30. Endothelial cells were exposed to experimental conditions and are imaged intact under a
microscope following antibody labeling of NF-κB, a protein located inside endothelial
cells. Select the following statement that best describes the approach that should be
used to image the endothelial cells in this experiment.
a. Cells are imaged under a transmission electron microscope.
b. Cells are imaged using phase-contrast in the light microscope.
c. Cells are imaged under a fluorescence microscope.
d. Cells are imaged under a scanning electron microscope.

QUESTION 4

A substance that reacts the same to light polarized in any direction is _________________.

a.

an isotope

b.

anisotropic

c.

isotropic

d.

divergent

QUESTION 5

The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.

a.

comparator

b.

comparison bridge

c.

spectroscope

d.

stage

QUESTION 6

A stereo microscope can best be described as _____________________.

a.

Two compound microscopes aligned so that they each see a slightly different part of an object.

b.

Two compound microscopes aligned with a comparison bridge.

c.

A compound microscope with two separate stages and one ocular.

d.

A compound microscope with two eyepieces and a camera mount.

QUESTION 7

In microscopy, resolution is a measure of ________________.

a.

The ability of the lenses to separate two tiny details that are close together.

b.

The total magnification power of the microscope.

c.

The empty magnification power of the microscope.

d.

The ability of an electron microscope to determine the presence of a large number of elements.

QUESTION 8

Two polarizers (polarizing filters) aligned 90° from each other will block out all light.

True

False

QUESTION 9

A simple convex lens creates a magnified real image of an object.

True

False

QUESTION 10

A trinocular microscope has three objective lenses mounted on a turret.

True

False

QUESTION 11

Liquids and gases are usually isotropic.

True

False

QUESTION 12

Match each of the following terms with Its Definition

Compound microscope a. Has a polarizer and an analyzer

Electron microscope b. Permits viewing two objects at once

Stereomicroscope c. Consists of a single convex lens

Comparison microscope d. Long working distance, three-dimensional

Simple hand magnifier e. Basic 2 convex lens microscope

Polarizing microscope f. Can magnify images more than 100,000 times

5. On a given microscope, the numerical apertures of thecondenser and low power objective lenses are 1.25 and 0.25,respectively. You are supplied with a filter that selects awavelength of 520nm.

a.) What is the limit of resolution on this microscope? Pleasebe specific to how you came up with this answer, what formula didyou use? b.) Will you be able to distinguish 2 points that are300nm apart as being separate, or will they blur into one?

6. on the same microscope as in Question#5, the high dryobjective lens has a numerical aperture of 0.85, what is the limitof resolution on this microscope, and will you be able todistinguish 2 points that are 300 nm apart as being separate, orwill they blur into one? Explain answer.

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