Log in
Chemistry
1
answer
0
watching
994
views
11 Nov 2019

Tris-Acetate EDTA (TAE) buffer is sold at 50X concentration. You will need 25 liters of this solution at a concentration of 1X for use during agarose gel electrophoresis in the lab. How would you make this solution?

Watch
1
answer
0
watching
994
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Jarrod Robel
Jarrod RobelLv2
2 Nov 2019

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share
discord banner image
Join us on Discord
Chemistry Study Group
Join now

Related textbook solutions

Basic Chemistry
5 Edition,
Timberlake
ISBN: 9780134138046
Principles of Chemistry Molecular Approach
4th Edition,
Tro
ISBN: 9780134112831
Chemistry: Structure and Properties
2nd Edition,
Tro
ISBN: 9780134293936
Principles of Chemistry Molecular Approach
3rd Edition, 2014
Tro
ISBN: 9780321971944
Chemistry: A Molecular Approach
3rd Edition,
Tro
ISBN: 9780321809247
Chemistry: A Molecular Approach
5th Edition,
Tro
ISBN: 9780134874371
Principles of Chemistry: A Molecular Approach
4th Edition,
Tro
ISBN: 9780134895741
Chemistry: The Central Science
14th Edition, 2017
Brown
ISBN: 9780134414232

Related questions

a) Calculate how you would make 1.0 L of 10x TBE (0.9 M Tris, 0.9 M Boric Acid, 20 mM EDTA) Tris MW=121.4 g/mole; Boric Acid MW=61.84 g/mole; EDTA MW=292.2 g/mole

b) For your polyacrylamide gel electrophoresis gel you need 50 mL of 0.5x TBE. Calculate and describe how you would make this solution using your stock solution.

4.) a.) Given a 25X stock solution of Tris Glycine SDS (i.e. running buffer in gel electrophoresis), what volumes of the stock and DI water should be combined to make 100 mL of a 1X Tris Glycine SDS solution?

b.) You have 1 mL of a previously prepared sample buffer to which you wish to add DTT (which is unstable in solution). What volume of 1M DTT will you need to add to achieve a final concentration of 50 mM DTT in the sample buffer?

You have a 10 mg/ml stock solution of ethidium bromode and want a final concentration of 1 ug/ml (microgram/mililiter) in 1 L of premade 1X TAE Buffer. Determine how much stock ethidium bromide and 1X TAE you will need to make the dilute solution. Please calculate the dilution factor and show steps using C1V1=C2V2

Weekly leaderboard

Home
Homework Help 3,900,000
Chemistry 830,000
Start filling in the gaps now
Log in