Determine the amounts/volumes of the chemicals needed to prepare the following solutions:
5. 100 ml of 0.1 N HCl from concentrated (12.0 M) HCl [you will be asked to make this solution as practice]
6. 100 ml of 1.0 M Tris pH = 7.6 MW of Tris base is 121 g/mole [you will be asked to make this solution as practice]
7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to need HCl or NaOH to adjust the pH to 7.0?
To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.
8. What is the minimum volume of 50X TAE that you will need to make?
Determine the amounts/volumes of the chemicals needed to prepare the following solutions:
5. 100 ml of 0.1 N HCl from concentrated (12.0 M) HCl [you will be asked to make this solution as practice]
6. 100 ml of 1.0 M Tris pH = 7.6 MW of Tris base is 121 g/mole [you will be asked to make this solution as practice]
7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to need HCl or NaOH to adjust the pH to 7.0?
To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.
8. What is the minimum volume of 50X TAE that you will need to make?
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