1. In what pH range can glycine be used as an effective buffer due to its amino groups?

you have two types of dialysis bags with molecular weight cut offs at 15kD and 25kD. Wich dialysis bag if any can be used to effectively dialyze the following mixtures against phosphate buffer to remove excess NaCl? Explain briefly

a. Protein A (mol Wt. 10kD) in phosphate buffer with 2M NaCl

b. Protein B (mol. Wt. 25kD) in phosphate buffer with 2M NaCl

Given a mixture of GIVE (AMINO ACIDS, GLYCINE, ISOLEUCINE, VALINE, GLUTAMIC ACID,) and TAKE (ALSO AMINO ACIDS), and buffers at pH 2,7 and 10, which buffer would you use to achieve their electrophoretic separation. Explain the answer based upon the charges on the two molecules.

What are the properties of amino groups, and why are they biologically

important?