Biology 2290F/G Study Guide - Final Guide: Calcium Chloride, Antimicrobial Resistance, Ampicillin
18 Apr 2017
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Tips for designing an experimental study to test a hypothesis: How spectronic 20 works: spectronic 20 does not measure the absorbance. Blank reference: a sample that contains all the substances except for the material whose absorbance you wish to measure. Transmittance is compared to a blank reference to see how much light has been absorbed by the sample. Any absorbance detected when the sample is read is only due to the material being measured. Beer"s law: absorbance of a solution is proportional to the number of absorbing particles (or concentration of the absorbing material) in solution. Lambert"s law: for a thin later of sample, the sample proportion of light is absorbed regardless of the radiant power of the incident light [io]. Spectrophotometry can be used to identify or characterize compounds in solution by producing an absorption spectrum. An absorption spectrum is a graph of the absorbance values of a chemical compound in solution measured at a series of different wavelengths.
