Biology 2290F/G Study Guide - Final Guide: Calcium Chloride, Difference Set, Plasmid
17 Apr 2016
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Explain the biology/chemistry underlying the various steps of plasmid transformation (i. e. explain the function of reagents such as calcium chloride, etc. ) In a cell wall there are about 300-400 openings. E. coli is gram negative so it has 2 membranes outer and inner. Where the two membranes join is called an adhesion zone. Long structures that stick out are called lippopolysacherides. May grab nutrients and other things in the structure. Dna has phosphate groups that are negatively charged. At the base of the lps there is also a negatively charged phosphate group. Movement of the lps is diminished making it easier for the plasmid to get past them. Sets up a big temperature difference before the heat pulse. Causes proteins to get blown out from the membrane opening up pores. Plasmid can get through the cell wall here. Every is entrapped in ice, heat pulse will allow for things to expand.
