01:119:117 Study Guide - Midterm Guide: Drag Equation, Sharps Waste, Absorbance
6 Apr 2017
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Handling the pipetman: press the plunger to the first stop before entering the liquid, slowly release plunger. Do not release the plunger too fast (liquid will shoot up into pipetman: use second stop only when dispensing liquid, always use a new pipet tip, never set a pipet above or below its range. How to make a specific volume of dilute from a stock: Dftotal = df1 x df2 x df3 . The practical is a time-based test don"t waste time finding averages for these absorbances. Your blank must be at least 1 ml and consists of your water/buffer. X-axis - concentration (independent variable - variable you manipulate) Y-axis - absorbance (dependent variable - observing what happens) Add a linear trendline to find the slope: make two columns for x (concentration) & y (absorbance) This info is from your serial dilution chart (conc. ) Make new columns for sample names,absorbance of unknowns, and. All but 1 concentration & all absorbances:
