BIOCHEM 2EE3 Chapter 4: Chapter 4

• School: McMaster University
• Department: Biochemistry
• Course: BIOCHEM 2EE3
• Professor: Felecia Vulcu

Document Summary

Clone: population of organisms or an organism that is genetically homogenous because they were derived from a single ancestor: some plasmids in bacterial cells are resistant to certain antibiotics due to a mutation in the genome, the antibiotic resistant gene is removed with restriction endonucleases: cut sticky or blunt ends at a specific, palindromic dna site, the gene is removed; the remainder of the cell is ligated back together with dna ligase, to form recombinant dna molecules, the antibiotic resistant gene which will be cloned is introduced into a vector, the vector is cleaved at the same restriction site (use same restriction enzyme) in step 2, vector is annealed via dna ligase, and brought into cells, which will replicate the exact genome = create clones, note: if the gene did code for a protein, success of gene cloning can be confirmed via mass spectrometry or reactivity with appropriate antibody.