BME 674 Chapter 5-10: Problem Set 2

1) Is it possible to use a 12% separating gel to run a set of proteins that range in MW from 10,000 to 250,000 Da? Explain.

2) You have an enzyme UTSase that is composed of three subunits. Two of the subunits are 34 kD and the other is 17 kD. How many bands will you see on an SDS-PAGE? Explain your answer.

3) What is the purpose of tracking dye in SDS-PAGE? What are the ingredients of sample buffer? Describe functions of each constituent.

CONTEMPORARY ISSUES IN GLOBAL HEALTH

Hey,here is the message below from professor.

Choose 1 topic about GLOBAL HEALTH and follow step below to complete report.

Students are required to do a simple but comprehensive research about a real global health problem that students are interested in. After that, students will analyze the current status/trend of the problem and generate innovative/possible solutions toward the problem. The article writing will be a 5-page, single-spaced, with Times New Roman and 12-point font size words article. The structure of article writing include: (1) Background, literature review and problem (~3 pages); (2) Discussion and Strategies/solutions (~2 pages).

Describe as much as you can.please provide References.Thank you for help!

1) Is it possible to use a 12% separating gel to run a set of proteins that range in MW from 10,000 to 250,000 Da? Explain in detail.

2) You have an enzyme UTSase that is composed of three subunits. Two of the subunits are 34 kD and the other is 17 kD. How many bands will you see on an SDS-PAGE? Explain your answer.

3) In paper electrophoresis, you separated amino acids based on charge. What is the variable this time? How do you know that there is only one variable in this separation?