BIOS-115 Chapter Notes - Chapter 2,12: Parvovirus B19, Erwin Chargaff, Histone H1

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Chapter 2 dna: the genetic material (p. 15-28) The composition and structure of dna and rna. Nucleotides = monomers that make up dna and rna: pentose sugar, nitrogenous base, & phosphate group, ribose rna, deoxyribose dna, sugar + base (no phosphate) = nucloside. Two classes of nitrogenous bases: purines: 9-membered, double ringed structure, adenine, guanine, pyrimidines: 6-membered, single ring structure, thymine, cytosine, uracil. Polynucleotides are linked by phosphodiester bonds: 5" to 3" linkages = polarity; 5" phosphate, 3" hydroxyl group. Different dna structures: a-dna, right-handed double helices, 11 bp/turn, only seen in low humidity, short and wide (22 diameter, narrow, deep major groove; shallow, minor groove. Dna in the cell: dna is in solution within the cell. Eukaryotic genome: organelles (mitochondria & chloroplasts) contain a single genome consisting of dna, main genome distributed among haploid set of chromosomes in the cell nucleus. Prokaryotic chromosomes: most prokaryotes contain a single, double stranded, circular dna chromosome.

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