BIOL 1208 Chapter : Biol Lab Report

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15 Mar 2019

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Document Summary

Diffusion is the movement of solutes from an area of high concentration to an area of lower concentration. The rate of diffusion is affected by numerous things including, the properties of the cell, the properties of the molecule being diffused, and the solution the cell is in. Hemolysis time is directly related to the rate of diffusion. In this experiment, we used bovine blood to measure the rate of the diffusion of various glycols. Glycols are polar molecules that belong to the alcohol family. Ethylene glycol, diethylene glycol and triethylene glycol have molecular weights of 62 g/mol, 106. 12 g/mol, and. We use bovine red blood cells to measure rate of diffusion. Cells placed in a hypertonic solution will shrivel and die. If a cell is in a hypotonic solution will rapidly swell and burst. The amount of time it takes for hemolysis to occur is directly related to the diffusion rate.

Related Questions

1. Microscopic cells or particles suspended in water exhibit atype of movement known as

_______________________________________________________________________.

2. The yeast cells in suspension appear to jiggle back and forthbecause they are bombarded

by the ______________ energy of water molecules which are inconstant, rapid, random

motion.

3. The movement of the molecules of a gas, liquid, or solid froma region of higher

concentration of that substance to a region of lesserconcentration is termed

____________________________.

4. What are 2 factors that influence the rate of diffusion?

5. ______________ is the diffusion of a solvent across aselectively permeable membrane.

6. In ______________________, water moves out of a plant cell byosmosis causing the

protoplast to shrivel up.

7. In __________________, water moves into an animal cell byosmosis causing the cell to

swell and burst.

8. The process of ________________ can be used in treatingkidney patients to separate

urea (small molecules) from proteins (large molecules) using aselectively permeable

membrane.

9. Which type of solution (hypotonic or hypertonic) is formed inthe soil environment

around root cells when a lawn is over fertilized? Would the rootcells lose or gain water?

10. Why is it common practice to place lettuce and celery inwater before serving them?

orchidbear657

Cell Membranes Lab Report

Diffusion

Compare the following terms: solvent, solute, andsolution.

Go to Lab, Section I, Exercise 1 to explore the factorsinfluencing the rate of diffusion and complete the following:

Predict how molecular mass and temperature may affect theoutcome of this experiment.

Record the data from information in the Lab in the tablebelow.

Time (min)	Diameter (mm)
Time (min)	5Â°C MB	5Â°C PP	23Â°CMB	23Â°C PP
0
15
30
45

Construct a line graph of the data from the table above. Yourgraph should include labeled axes, units, and a legend indicatingwhich treatment is presented. Sign, date and prepare an image ofyour graph and include it with this lab report.

Osmosis

Go to Lab, Section II, Exercise 2 to view a demonstration ofosmosis and answer the following questions:

Describe the net movement of water in osmometer 1.

How is the movement of water molecules related to theconcentration gradient of the solution?

Go to Lab, Section II, Exercise 3 to observe the effect ofsolute concentration on the rate of osmosis. Answer the followingquestions:

Was the net movement of water in bags 1 to 4 into or out of thebags?

Explain the results from bags 4 and 5.

Selective Permeability of Membranes

Go to Lab, Section III, Exercise 4 to learn about theimportance of selectively permeable membranes, then complete thetable and answer the following questions:

	Contents of Beaker
	0 Min	30 Min
Starch
Chloride ion

Which substances diffused through the dialysis membrane?

How does dialysis tubing model the selective permeability of aplasma membrane?

Tonicity

Go to Lab, Section IV, Exercise 5 and 6 to observe plasmolysisin Elodea cells and tonicity in red blood cells. Summarizethe concept of tonicity using blood and Elodea cells asexamples. Your description should incorporate these terms: turgid,plasmolysis, hemolysis, and crenation.

Cells	Isotonic Solution	Hypertonic Solution	Hypotonic Solution
Elodea
RBC

Based on your previous work, reproduce the table below and drawan illustration of a single cell in each box. Include arrows toshow the direction of water flow relative to each cell and dots tosymbolize solutes. Sign, date and prepare an image of your drawingand include it with this lab report.

Cells	Isotonic Solution	Hypertonic Solution	Hypotonic Solution
Elodea
RBC

Summary Questions

Compare diffusion and osmosis. Give an example of each.

2. In which direction will osmosisoccur if a 15% sugar solution is separated from a 25% sugarsolution by a selectively permeable membrane?

3. Why did osmosis, but not diffusionof sucrose molecules, occur across the dialysis membrane containing20% sucrose solution?

4. You are having a party and thecelery is limp. What might you do to make the celery crisp (turgid)again? What will occur in the cells?

5. A small amount of fertilizer(mineral salts) will stimulate plant growth, but over fertilizationcan kill plants? Why?

6. Michael adds sugar to his coffee.Explain what in this drink is the solvent, solute, and solution.

7. You add a cube of sugar to yourdrink. How could you speed up the diffusion of sugar moleculeswithout stirring?

8. How is dynamic equilibriumestablished within a solution?

amberdeer449

The option to feel out every sentense are: cell wall, increasing, chloroplasts, hypotonic, hypertonic, cytokinesis, plasmolysis, and decreasing

In an isotonic solution in animal cells, there is no net movement of water.

An animal cell in a ____________ solution may lyse or burst open.

An animal cell in a ____________ solution will shrivel up or crenate.

Plant cells react differently to osmotic changes due to the presence of the ____________ , a structure that animal cells lack.

For a plant cell in a ____________ solution, vacuoles fill with water, ____________ turgor pressure.

For a plant cell in a ____________ solution, the cytoplasm shrinks causing ____________ and ____________ turgor pressure.

------------------

The opcions to fell our the following sentenses are: mitochondria, chloroplasts, ATP, passive, against, carrier, small, receptor, large, with, and active

In contrast to ____________ transport, ____________ transport requires energy for molecules to pass through cell membranes.

This might occur when molecules need to move ____________ a concentration gradient, as is the case of thyroid cells where iodine collects in ____________ concentrations.

This type of transport requires both chemical energy in the form of ____________ and ____________ proteins.

Cells involved in this type of transport often have high numbers of ____________ in order to facilitate this process.

BIOL 1208 Chapter : Biol Lab Report

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